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1


Blast node

NetBlastP - compare an amino acid query sequence against
the NCBI GenBank "nr" protein sequence database


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Input
fasta amino acids file
Load a local file with data

Alternatively, paste here
Example

NetBlastTN - compare a protein query sequence against
the NCBI GenBank "nr" nucleotide sequence database using
dynamic translation in all reading frames


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Input
fasta amino acids file
Load a local file with data

Alternatively, paste here

2


Blast to multi fasta node

Blast2multifasta - Extract fasta sequences from
blast-generated xml file


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Input
blast xml file
Load a local file with data

Alternatively, paste here
HSP length
Minimum HSP length value (in percent)
Score
Minimum score value
E-value
Maximum evalue value
Percent identity
Minimum percent identity value
Use HSP length option
Use HSP length option
Use score option
Use score option
Use evalue option
Use evalue option
Use percent identity option
Use percent identity option

3


Attach sequences node

AttachSeqNet - Attach sequences (network version),
usefull program for protein sample enlargement.


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Input
fasta amino acids file
Load a local file with data

Alternatively, paste here
Attach to begin
Number of bases to attach to begin of sequences
Attach to end
Number of bases to attach to end of sequences

4


BLOSUM matrix generator node

BLOSUM - Generate BLOSUM matrix from protein multifasta file
using BLIMPS package (Henikoff et al., 1995)


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Input
Protein multifasta file
Load a local file with data

Alternatively, paste here
Example
Percent of identity
Minimum percent identity value


Pipeline
scheme