Start:
Blast node
Blast to multi fasta node
Attach sequences node
BLOSUM matrix generator node
Stop:
Blast node
Blast to multi fasta node
Attach sequences node
BLOSUM matrix generator node
1
Blast node
NetBlastP
- compare an amino acid query sequence against
the NCBI GenBank "nr" protein sequence database
Show/Hide input files
Input
fasta amino acids file
Load a local file with data
Alternatively, paste here
Example
NetBlastTN
- compare a protein query sequence against
the NCBI GenBank "nr" nucleotide sequence database using
dynamic translation in all reading frames
Show/Hide input files
Input
fasta amino acids file
Load a local file with data
Alternatively, paste here
2
Blast to multi fasta node
Blast2multifasta
- Extract fasta sequences from
blast-generated xml file
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Input
blast xml file
Load a local file with data
Alternatively, paste here
HSP length
Minimum HSP length value (in percent)
Score
Minimum score value
E-value
Maximum evalue value
Percent identity
Minimum percent identity value
Use HSP length option
Use HSP length option
Use score option
Use score option
Use evalue option
Use evalue option
Use percent identity option
Use percent identity option
3
Attach sequences node
AttachSeqNet
- Attach sequences (network version),
usefull program for
protein
sample enlargement.
Show/Hide input files
Input
fasta amino acids file
Load a local file with data
Alternatively, paste here
Attach to begin
Number of bases to attach to begin of sequences
Attach to end
Number of bases to attach to end of sequences
4
BLOSUM matrix generator node
BLOSUM
- Generate BLOSUM matrix from
protein
multifasta file
using BLIMPS package
(Henikoff et al., 1995)
Show/Hide input files
Input
Protein multifasta file
Load a local file with data
Alternatively, paste here
Example
Percent of identity
Minimum percent identity value
Pipeline
scheme